In vitro hydrogen production by glucose dehydrogenase and hydrogenase

1 July 1996

journal article
research article
Published by Springer Nature in Nature Biotechnology

Vol. 14 (7) , 872-874
https://doi.org/10.1038/nbt0796-872

Abstract

A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity.

Keywords

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