2-DIMENSIONAL IMMUNOELECTROPHORETIC PATTERN OF LOW-DENSITY AND VERY-LOW-DENSITY LIPOPROTEINS, WITH PARTICULAR REFERENCE TO FREDRICKSONS TYPE -3

  • 1 January 1977
    • journal article
    • research article
    • Vol. 23  (2) , 186-194
Abstract
The pattern of human serum low-density lipoproteins (LDL) and very-low-density lipoproteins (VLDL) as revealed by 2-dimensional (crossed) immunoelectrophoresis in hyperlipoproteinemia types II, III and IV, preliminarily diagnosed by means of a complex lipoprotein profile examination, is reported. The higher affinity of Oil Red stain for VLDL- than for LDL-immunoprecipitation zones is peculiar to this lipoprotein class, and crossed immunoelectrophoretograms may thus be helpful for laboratory diagnosis of types IIb and IV in problematic cases. The position and the medium affinity for Oil Red of broad beta zones appearing in immunoelectrophoretograms of type III hyperlipoproteinemia are suggested as useful criteria for the diagnosis of this condition. From the structural and staining characteristics of VLDL-immunoprecipitation zones, alteration of both composition and antigenic properties of VLDL in hyperlipoproteinemias IIb-IV is deduced.

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