Comparison of Two Methods for Analysis of Aflatoxin M1 in Milk
Open Access
- 1 March 1977
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 60 (3) , 450-452
- https://doi.org/10.3168/jds.s0022-0302(77)83886-1
Abstract
The cellulose chromatography method of the Association of Official Analytical Chemists for determination of aflatoxin M1 [produced by Aspergillus flavus and related organisms] was compared with a 2 dimensional TLC procedure. The minimum detectable aflatoxin M1 by the Official procedure was approximately .01 .mu.g/kg while the TLC procedure has a claimed sensitivity of .004 .mu.g/kg. Analyses of milk samples by TLC agreed with results of the Official procedure in the range of .01-.25 .mu.g/l. Each method consistently yielded recoveries greater than 87%, and there was no significant difference between means or variances of each method. The TLC method took less time than the Official method and showed a reproducible lower threshold sensitivity of .005 .mu.g/l.This publication has 9 references indexed in Scilit:
- Stability of Aflatoxin M in MilkJournal of Dairy Science, 1975
- Determination of aflatoxin M1 in milk at the parts per trillion levelJournal of Chromatography A, 1975
- Hepatic Carcinogenesis of Aflatoxin M1 in Rainbow Trout (Salmo gairdneri) and Its Enchancement by Cyclopropene Fatty Acids23JNCI Journal of the National Cancer Institute, 1974
- Implication of mycotoxins for human healthJournal of Agricultural and Food Chemistry, 1974
- Consumption and fate of aflatoxin B1 by lactating cowsJournal of Agricultural and Food Chemistry, 1974
- Method for the Determination of Aflatoxin M1 in Fluid Milk and Milk ProductsJournal of AOAC INTERNATIONAL, 1973
- Excretion of aflatoxin in a lactating cowFood and Cosmetics Toxicology, 1968
- Toxic Metabolites of Aspergillus FlavusNature, 1963
- Toxicity associated with Certain Samples of GroundnutsNature, 1961