RADIOIMMUNOASSAY OF LEUCINE-ENKEPHALIN-LIKE SUBSTANCE IN HUMAN AND CANINE PLASMA

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Abstract
Fasting human plasma immunoreactive leu-enkephalin (ir-leu-enkephalin) measured by radioimmunoassay averages 54±10 pg/ml. The method depends on acidification of plasma to protect against destruction of the peptide, adsorption to XAD-2 resin, extraction from the resin by aqueous methanol and concentration by evaporation. Plasma enkephalin in the dog increased from 13 to 56 pg/ml following insulin-induced hypoglycemia.