CHEMICAL AND BIOLOGICAL PROPERTIES OF A LIPOPOLYSACCHARIDE OF BCG RESPONSIBLE FOR HEMAGGLUTINATION REACTION

Abstract
In the previous paper the author and her collaborators reported the isolation and purification of hemagglutination antigen of Mycobacterium tuberculosis Aoyama B (virulent human type) by zone electrophoresis and ultracentrifugation techniques (Tsumita, Matsumoto and Mizuno, 1959, 1960) . They isolated a macromolecular lipopolysaccharide which was electrophoretically and ultracentrifugally homogenous (26 S) . The component sugars were mannose and arabinose. It gave a high titer of hemagglutination reaction against anti-Aoyama B horse serum as well as against anti-BCG rabbit serum. The present author intended to isolate and purify a hemagglutination antigen from BCG similar to that of Aoyama B strain and to elucidate as to whether it crosses with anti-Aoyama B serum or not. A lipopolysaccharide composed of mannose, arabinose and glucose was isolated, giving a high titer in hemagglutination reaction both for anti-Aoyama B and anti-BCG sera.

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