The fermentation, isolation and characterization of macromolecular peptide antibiotics: AN-7A, -7B and -7D.

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Abstract
A group of new macromolecular peptide antibiotics, named AN-7, was isolated from the culture broth of Streptomyces griseoincarnatus AJ9424. AN-7 was fractionated into 3 different components, A, B and D. From 18 l of culture broth (78 U/ml), 10 mg of AN-7A with a specific activity of 2053 U/mg, 9 mg of AN-7B (1167 U/mg) and 11 mg of AN-7D (6225 U/mg) were obtained. All of the samples gave single bands on polyacrylamide gel electrophoresis. They were acidic polypeptides with MW of 12,400-13,000. Their UV absorption spectra showed maximal peaks at 280 nm and shoulders at 290 nm. Each AN-7 component had a nonprotein chromophoric component. AN-7A, -7B and -7D had no activity against the gram-negative bacteria tested but strongly inhibited the growth of gram-positive bacteria and Escherichia coli MP2, a macromolecule-permeable mutant strain. The AN-7 components were mutagenic. These antibiotics inhibited the in vitro growth of L1210 [mouse leukemia] cells (ED50 0.13-0.18 .mu.g/ml). AN-7A, -7B and -7D also inhibited the growth of L1210 cells in mice.

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