3-methyladenine-DNA-glycosylase and O6-alkyl guanine-DNA-alkyltransferase activities and sensitivity to alkylating agents in human cancer cell lines
Open Access
- 1 April 1996
- journal article
- Published by Springer Nature in British Journal of Cancer
- Vol. 73 (7) , 861-865
- https://doi.org/10.1038/bjc.1996.153
Abstract
The activities and the expression of 3-methyladenine glycosylase (3-meAde gly) and O6-alkylguanine-DNA-alkyltransferase (O6 ATase) were investigated in ten human cancer cell lines. Both 3-meAde gly and O6 ATase activities were variable among different cell lines. mRNA levels of the O6 ATase gene, appeared to be related to the content of O6 ATase in different cell lines, whereas no apparent correlation was found between mRNA of 3-meAde gly and the enzyme activity. No correlation was found between the activity of the two enzymes and the sensitivity to alkylating agents of different structures such as CC-1065, tallimustine, dimethylsulphate (DMSO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), cis-diamminedichloroplatinum (cDDP) and melphalan (L-PAM). The most striking finding of this study is that a correlation exists between the activity of O6 ATase and 3-meAde gly in the various cell lines investigated (P<0.01), suggesting a common mechanism of regulation of two DNA repair enzymes.Keywords
This publication has 41 references indexed in Scilit:
- DNA sequence-specific adenine alkylation by the novel antitumor drug tallimustine (FCE 24517), a benzoyl nitrogen mustard derivative of distamycinNucleic Acids Research, 1995
- DNA damage tolerance, mismatch repair and genome instabilityBioEssays, 1994
- Evidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cellsCarcinogenesis: Integrative Cancer Research, 1994
- Adaptation to alkylation resistance involves the induction of a DNA glycosylaseNature, 1982
- Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agentsNature, 1982
- Repair of O6-ethylguanine in DNA by a chromatin fraction from rat liver: transfer of the ethyl group to an acceptor protein.Proceedings of the National Academy of Sciences, 1981
- Repair of alkylated DNA in Escherichia coli. Methyl group transfer from O6-methylguanine to a protein cysteine residue.Journal of Biological Chemistry, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Two enzymes are required for strand incision in repair of alkylated DNANature, 1977
- Possible Relevance of O–6 Alkylation of Deoxyguanosine to the Mutagenicity and Carcinogenicity of Nitrosamines and NitrosamidesNature, 1969