Homogeneous Amperometric Immunoassay for Digoxin
- 1 October 1994
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 27 (13) , 2443-2453
- https://doi.org/10.1080/00032719408002653
Abstract
A homogeneous spectrophotometric EMIT immunoassay for the measurement of digoxin has been converted to an amperometric immunoassay format capable of analysing digoxin in diluted serum. NADH produced by glucose-6-phosphate dehydrogenase (G6PDH) labelled digoxin was detected amperometrically by its rapid, efficient oxidation at platinized activated carbon electrodes (PACE) poised at +150 mV vs Ag/AgCl. The new amperometric procedure resulted in a simplified assay protocol with a decreased overall assay time, enhanced NADH detection and minimal sample pretreatment.Keywords
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