Demonstration of -Oxidation and Acetoacetate Formation in a Mouse Hepatoma.

Abstract
Slices of a transplanted mouse hepatoma. C954, oxidized octanoate-1-C14 to c14O2 and acetoacetate-l,3-C14. Isotopic acetoacetate accumulated in quantities approaching that recovered in respiratory CO2. The distribution of isotope in the ketone body is consistent with the view that the short-chain fatty acid was degraded by beta-oxidation, the resulting C2 fragments condensing to form acetoacetate as well as being oxidized to CO2.