Ampicillin‐dextrin agar medium for the enumeration of Aeromonas species in water by membrane filtration
- 11 March 1987
- journal article
- research article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 62 (3) , 279-287
- https://doi.org/10.1111/j.1365-2672.1987.tb02410.x
Abstract
Published selective media were evaluated for the isolation of Aeromonas spp. from environmental samples by membrane filtration. Satisfactory recoveries were obtained only with mA agar (Rippey & Cabelli) and dextrin‐fuchsin‐sulphite agar (Schubert), but neither was sufficiently selective. The positive aspects of these two media were combined in a new medium, ampicillin‐dextrin agar. Recovery from pure cultures and environmental samples was optimal at an ampicillin concentration of 10 mg/l and incubation for 24 h at 30°C under aerobic conditions, and specificity was high (i.e. confirmation rate usually Aeromonas spp. from sea water provided that the vibriostatic agent 0/129 is added at 50 mg/1.Keywords
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