Identification of 11,13-dihydroxy-14-octadecaenoic acid as a circulating Na+/K+-ATPase inhibitor

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Abstract
Two digitalis-like compounds (DLC) were purified to homogeneity from bovine plasma. The purification procedure consisted of organic extractions and batch chromatography followed by three subsequent separations using reverse-phase high-performance liquid chromatography. The presence of a DLC in the different fractions was monitored by their ability to inhibit (a) [3H]ouabain binding to rat brain synaptosomes, and (b) microsomal Na+/K+-ATPase activity. Using mass spectrometry and nuclear magnetic resonance spectroscopy the structure of one of the DLCs was identified as 11,13-dihydroxy-14-octadecaenoic acid. It is suggested that this new hydroxy, unsaturated, fatty acid derivative may regulate Na+/K+-ATPase activity under some physiological and pathological conditions. Journal of Endocrinology (1991) 128, 71–78

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