Partition functions of unit-copy plasmids can stabilize the maintenance of plasmid pBR322 at low copy number
Open Access
- 1 November 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 168 (2) , 1010-1013
- https://doi.org/10.1128/jb.168.2.1010-1013.1986
Abstract
The maintenance of plasmid pBR322 is highly unstable in a polA12 strain of Escherichia coli at 29 degrees C due to severely reduced copy number. Under these conditions, introduction of the par (partition) locus of plasmid P1 or the par (sop) region of F into pBR322 stabilizes it. A region with similar activity was detected in the P7 plasmid. The activity of the P1 par locus was dependent on the P1 parA gene product and was sensitive to par-specified incompatibility.This publication has 23 references indexed in Scilit:
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