The Enzymic Formation of a Methylenedioxyphenyl Derivative Exhibiting an Isocyanide-like Spectrum with Reduced Cytochrome P-450 in Hepatic Microsomes
- 1 January 1971
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 1 (6) , 581-591
- https://doi.org/10.3109/00498257109112269
Abstract
1. Methylenedioxyphenyl compounds (piperonyl butoxide, methylene-dioxybenzene, isosafrole) were metabolized by rat liver microsomes in the presence of oxygen and NADPH (but not NADH) to give products which exhibited an isocyanide-like absorption spectrum with reduced cytochrome P-450 of liver microsomes. 2. The presence of hexobarbital inhibited the formation of the isocyanide-like spectrum, presumably by inhibiting the formation of the metabolite. 3. The optical spectrum absorption characteristics of the complex of the metabolite with reduced cytochrome P-450 varied with changes in pH. 4. The metabolite, or the metabolite-cytochrome P-450 complex formed, was sufficiently stable to survive the oxidation and re-reduction of the microsomal cytochrome P-450.Keywords
This publication has 14 references indexed in Scilit:
- On the inhibitory action of mersalyl on microsomal drug oxidation: A rigid organization of the electron transport chainArchives of Biochemistry and Biophysics, 1971
- Microsomal electron transport reactionsArchives of Biochemistry and Biophysics, 1971
- Properties of housefly microsomal cytochromes in relation to sex, strain, substrate specificity, and apparent inhibition and induction by synergist and insecticide chemicalsLife Sciences, 1970
- Mixed-function oxidase involvement in the biochemistry of insecticide synergistsJournal of Agricultural and Food Chemistry, 1970
- Importance of demethylenation in the metabolism in vivo and in vitro of methylenedioxyphenyl synergists and related compounds in mammalsBiochemical Pharmacology, 1970
- Environmental aspects of methylenedioxyphenyl and related derivativesEnvironmental Research, 1969
- Metabolism in relation to mode of action of methylenedioxyphenyl synergists in housefliesJournal of Agricultural and Food Chemistry, 1969
- Anomalous Spectral Interactions of Reduced P-450 with Ethyl Isocyanide and Some Other Lipophilic Ligands*The Journal of Biochemistry, 1967
- Evidence for two forms of P-450 hemoprotein in microsomal membranesBiochemical and Biophysical Research Communications, 1966
- Biochemistry of Drug Oxidation and Reduction by Enzymes in Hepatic Endoplasmic ReticulumPublished by Elsevier ,1966