High-speed Chromatographic Determination of Ascorbic Acid Derivatives. I. Rapid Analysis of Ascorbic Acid 2-Sulfate
Open Access
- 1 January 1977
- journal article
- research article
- Published by Pharmaceutical Society of Japan in YAKUGAKU ZASSHI
- Vol. 97 (1) , 106-108
- https://doi.org/10.1248/yakushi1947.97.1_106
Abstract
A rapid estimation method utilizing high-speed liquid chromatography is described for determination of ascorbic acid 2-sulfate (AsS). Separation can be achieved within 6 min employing a 1-m column packed with Zipax SAX using 0.0005 M Na2B4O7-0.05 M NaClO4 (93:7 vol/vol) (pH 7.0) solution as an eluant in a Du Pont LC 830 liquid chromatograph. Typical chromatograms obtained by this method are shown. Precision in this method was less than .+-. 2.0% with the relation Y = 0.31X .+-. 0.07 and a relative coefficient of 0.999. The limit of detection was 40 ng. AsS from human urine was examined by this analytical method. The peak component was identified by UV absorption spectra and TLC as AsS. .Keywords
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