Fluorescence properties of the Ca2+Mg2+‐ATPase protein of sarcoplasmic reticulum labeled with 7‐chloro‐4‐nitrobenzo‐2‐oxa‐1,3‐ diazole
- 1 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 259 (2) , 254-256
- https://doi.org/10.1016/0014-5793(90)80021-a
Abstract
The fluorescence intensity of the Ca2+,Mg2+-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 μM calcium. The observed enhancement indicates that the fluorophore, NBD-Cl, can detect conformational changes in the ATPase protein.Keywords
This publication has 37 references indexed in Scilit:
- Synthesis of spin‐labeled 2‐azido‐ATP: Evidence for distinct nucleotide‐binding sites in calcium pump protein from sarcoplasmic reticulumFEBS Letters, 1989
- Evidence for the cytoplasmic location of the N- and C-terminal segments of sarcoplasmic reticulum (Ca2+Mg2+)-ATPaseBiochemical and Biophysical Research Communications, 1989
- Characterization of a cysteine‐containing peptide after affinity labelling of Ca2+‐ATPase of sarcoplasmic reticulum with the disulfide of 3′(2′)‐O‐biotinyl‐thioinosine triphosphateEuropean Journal of Biochemistry, 1989
- Characterization of the tryptophan fluorescence from sarcoplasmic reticulum adenosine triphosphatase by frequency-domain fluorescence spectroscopyBiochemistry, 1989
- Modification of the (Ca2 + Mg2)-ATPase protein of sarcoplasmic reticulum with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazoleBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1989
- Two Ca2+ ATPase genes: Homologies and mechanistic implications of deduced amino acid sequencesCell, 1986
- Site‐Directed Fluorogenic Modification of Bacteriophodopsin by 7‐Chloro‐4‐nitrobenz‐2‐oxa‐1,3‐diazoleEuropean Journal of Biochemistry, 1983
- Excimer Formation of ATPase from Sarcoplasmic Reticulum Labeled with N‐(3‐Pyrene)maleinimideEuropean Journal of Biochemistry, 1983
- Reaction of (sodium(1+)-potassium(1+) ion)-activated ATPase with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: evidence for an essential tyrosine at the active siteBiochemistry, 1978
- The reactivity of SH groups with a fluorogenic reagentFEBS Letters, 1970