Single DNA Rotaxanes of a Transmembrane Pore Protein

Abstract
Threading a molecular needle: An α‐hemolysin transmembrane pore can be threaded in a desired orientation by DNA–poly(ethylene glycol) (DNA‐PEG) hybrid strands to yield functional rotaxanes (see figure). The single‐molecule rotaxanes are stable and have a reversible two‐state switching capacity that depends on the applied potential, orientation, and method of threading and capture used.