Chromosome Constitution and Gamma-Ray Sensitivity: A Possible Correlation in Hamster Cells Cultured in Vitro

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Abstract
Survival estimates were made for two sublines of Chinese hamster cells cultured in vitro, designated clones D and F, which had a preponderance of cells with 23 and 46 chromosomes, respectively. A digital computer was programmed to fit the best multitarget survival curves to the data by the method of least squares. The slopes of the survival curves for the two cell lines were similar, but the cell line with the largest number of chromosomes exhibited a larger extrapolation number; 8.5 for clone F, compared with 2.95 for clone D. Split-dose experiments were also performed. The recovery factor (defined to be the number of colonies formed on plates receiving the split dose, divided by the number of colonies formed on plates receiving the same total dose in a single exposure) was larger for cells of clone F than for cells of clone D-8.5, as opposed to 4.4. These results tend to confirm previous reports in the literature that radiation response varies with chromosome number, cells of higher ploidy being more resistant.

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