Role of RNA transcripts in replication incompatibility and copy number control in antibiotic resistance plasmid derivatives
- 1 April 1981
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 290 (5809) , 794-797
- https://doi.org/10.1038/290794a0
Abstract
The genes required for autonomous replication and incompatibility in the antibiotic resistance plasmids R100 and R1 have been located within a 2.5-kilobase region of the 90-kilobase genome, within which the incompatibility gene occupies a 1.3-kilobase region excluding the replication origin. We now report that three RNA species are synthesized in vitro from the 2.5-kilobase region, which R100 and R1 have in common. One, a long RNA molecule which is transcribed in the direction of DNA replication, probably acts as a messenger or a protein required for plasmid replication. The second RNA species, only 91 nucleotides long, is transcribed in the opposite direction, from a region of the DNA entirely contained within the first and known to specify incompatibility and copy control functions. The third RNA species, 150 bases long, is transcribed from a region including the replication origin1; it may be a primer of DNA synthesis or, in conjunction with the second of the three RNA species, an influence in the control of replication.Keywords
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