Effects of Certain Triazenes on Chick Embryos and on Tumors Explanted to the Chorioallantois.

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Abstract
3,3-Dimethyl-l-phenyltriazene, "triazene", was tested for its teratogenic action in the chick embryo and for its effect on mouse and human tumors explanted to the chorioallantois of the chick embryo. The teratogenic syndrome produced by the injection of "triazene" into the yolk sac of the 4-day chick embryo consisted of parrot-beak, micromelia, bent tibiotarsus, edema, and feather growth inhibition. This syndrome was prevented by a supplemental injection of nicotinamide. Several analogs of "triazene" were also tested for teratogenic properties. Following injections into the 12-day yolk sac, "triazene" caused stunting, feather inhibition, edema and a reduction in spleen weight, but no skeletal abnormalities. When injected into the yolk sac of embryos carrying chorioallantoic grafts of mouse sarcoma 180 and Toolan''s human epidermoid carcinoma no. 3, "triazene" inhibited the growth of the tumors, caused cellular enlargement, nuclear changes indicative of cellular injury, and a marked decrease in the-number of cells. Effects on the tumors and the 12-day embryo are similar to those following treatment with polyfunctional alky 1 ating agents, whereas the effects on the 4-day embryos resemble those produced by insulin, sulfanilamide, eserine and certain diazonium salts.