Chloride‐selective channels of large conductance in bovine aortic endothelial cells

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Abstract
Single‐channel currents of an anionic channel in the plasma membrane of cultured bovine aortic endothelial cells have been recorded with the patch‐clamp technique. The channel is selective for chloride over cations, and has an average single channel conductance of 382 picosiemens in symmetric 140 millimoles of chloride. In addition to the main conductance state it shows well‐defined subconductance states of about 50, 100, 150 and 200 picosiemens. The channel is very active at membrane potentials close to 0 mV, but steps to either positive or negative membrane potentials above ± 20 millivolt lead to a rapid inactivation of the channel. Changes in the concentrations of free calcium or andenosine tri‐phosphate on the cytosolic surface do not influence channel activity. The chloride channel rarely opens at resting membrane potential, but it may help repolarize endothelial cells following depolarizing stimuli.