The use of enzyme electrophoresis for the identification of the species of Eimeria present in field isolates of coccidia
- 1 December 1982
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 85 (3) , 437-442
- https://doi.org/10.1017/s0031182000056213
Abstract
The electrophoretic mobility of glucose phosphate isomerase (GPI) was used to identify E. acervulina, E. praecox and E. mivati in isolates of coccidia [in chickens] from commercial sites in the UK. Mixtures of 2 or 3 spp. gave separate bands upon electrophoresis, and each species could be identified provided that it formed at least 10-20% of the total number of oocysts present in a sample of 8 .times. 106 oocysts. The principal forms of GPI corresponded to the GPI of E. acervulina (H), E. praecox (H) and E. mivati (W) and were found in 38, 34 and 8 isolates, respectively, of the 46 examined. Three further variants of GPI are described.This publication has 15 references indexed in Scilit:
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