Orientation of functional activating regions in the Escherichia coli CRP protein during transcription activation at class II promoters
Open Access
- 1 March 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 24 (6) , 1112-1118
- https://doi.org/10.1093/nar/24.6.1112
Abstract
At class II CRP-dependent promoters the DNA site for CRP overlaps the DNA site for RNA polymerase, covering the −35 region. Transcription activation at class II CRP-dependent promoters requires a contact between an activating region in the upstream subunit of the bound CRP dimer and a contact site in the C-terminal domain of the α-subunit of RNA polymerase. Transcription activation is suppressed by amino acid substitutions in the activating region, but activation can be restored by second site substitutions at K52 or E96. These substitutions identify two separate regions on the surface of CRP that appear to be able to interact with RNA polymerase specifically at class II promoters. Using the method of ‘oriented heterodimers’ we show that these alternative activating regions are functional in the downstream subunit of the bound CRP dimer.Keywords
This publication has 24 references indexed in Scilit:
- Evidence for Contact between the Cyclic AMP Receptor Protein and the σ70 Subunit of Escherichia coli RNA PolymerasePublished by Elsevier ,1995
- Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP) II. Role at class I and class II CAP-dependent promotersJournal of Molecular Biology, 1994
- Domain organization of RNA polymerase α subunit: C-terminal 85 amino acids constitute a domain capable of dimerization and DNA bindingCell, 1994
- Location and orientation of an activating region in the Escherichia coli transcription factor, FNRMolecular Microbiology, 1994
- Role of the Sigma70 Subunit of Escherichia coli RNA Polymerase in Transcription ActivationJournal of Molecular Biology, 1994
- Interactions between the Escherichia coli cyclic AMP receptor protein and RNA polymerase at Class II promotersMolecular Microbiology, 1993
- Transcription activation at Class I CAP‐dependent promotersMolecular Microbiology, 1993
- Identification of the functional subunit of a dimeric transcription activator protein by use of oriented heterodimersCell, 1993
- FNR and its role in oxygen-regulated gene expression inEscherichia coliFEMS Microbiology Letters, 1990
- RNA polymerase makes important contacts upstream from base pair −49 at the Escherichia coli galactose operon P1 promoterGene, 1987