Abstract
The results of a biological simulation study comparing the method of maximum likelihood for binary regression with unreplicated data and 2 approximate methods are presented and discussed. The 2 approximate methods are that of Cox and unweighted least squares with the 0''s replaced by -3 and the 1''s by +3. With 20 or more data values the maximum likelihood methods worked quite well but with fewer than 20 the approximate methods are to be preferred.

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