Isolation and characterization of microsatellites from the canine genome
- 1 August 1993
- journal article
- Published by Wiley in Animal Genetics
- Vol. 24 (4) , 289-292
- https://doi.org/10.1111/j.1365-2052.1993.tb00313.x
Abstract
Microsatellite sequences comprising (dC-dA)n.(dG-dT)n repeats have been isolated from canine libraries and sequenced. Oligonucleotide primers have been synthesized to the microsatellite flanking sequences and used in the polymerase chain reaction to amplify those loci from genomic DNA. The degree of polymorphism of each microsatellite was estimated in a set of unrelated dogs. It is concluded that of the 10 loci studied, nine are sufficiently polymorphic to be useful in genetic studies.Keywords
This publication has 10 references indexed in Scilit:
- Cloning of highly polymorphic microsatellites in the horseAnimal Genetics, 1992
- Variable (dG-dT)n·(dC-dA)n sequences in the porcine genomeGenomics, 1992
- ‘Touchdown’ PCR to circumvent spurious priming during gene amplificationNucleic Acids Research, 1991
- Evolution and distribution of (GT)n repetitive sequences in mammalian genomesGenomics, 1991
- Genetic analysis of autoimmune type 1 diabetes mellitus in miceNature, 1991
- Systematic cloning of human minisatellites from ordered array charomid librariesGenomics, 1990
- Informativeness of human (dC-dA)n · (dG-dT)n polymorphismsGenomics, 1990
- Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellitesNucleic Acids Research, 1990
- Hypervariability of simple sequences as a general source for polymorphic DNA markersNucleic Acids Research, 1989
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982