Outbreak of Infection WithAcinetobacterStrain RUH 1139 in an Intensive Care Unit

Publisher Website
Google Scholar
Abstract
Objective.: To investigate a nosocomial outbreak of infection withAcinetobacterstrain RUH 1139, in the unit of high neonatal risk at University Hospital of The Andes (Mérida, Venezuela).Methods.: Twenty-eightAcinetobacterstrains were detected by biochemical testing and further identified to the species level by examination of the gene encoding 16S ribosomal DNA, using restriction analysis and gene sequencing. The epidemiological relationship between the strains was established by means of repetitive extragenic palindromic polymerase chain reaction (REP-PCR) and pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibilities were determined by disk diffusion.Results.: The spread of an epidemic strain ofAcinetobacterRUH 1139 among 16 patients over a period of 3 months was demonstrated using antimicrobial susceptibility testing, PFGE, and REP-PCR. The epidemic strain was also isolated in 2 of the sampled parenteral nutrition solutions. All the patients involved in the infection outbreak had received parenteral solution. Moreover, strains ofAcinetobacterRUH 1139 with another PFGE pattern and ofA. baumanniiwere sporadically isolated before and during the outbreak.Conclusion.: This is the first description of an outbreak of infection with this genospecies ofAcinetobacterin which parenteral nutrition solution was potentially the infection source.

This publication has 19 references indexed in Scilit: