HLA‐DR/Dw MATCHING BY PCR FINGERPRINTING: THE ORIGIN OF PCR FINGERPRINTS AND FURTHER APPLICATIONS
- 1 February 1991
- journal article
- Published by Wiley in International Journal of Immunogenetics
- Vol. 18 (1-2) , 147-153
- https://doi.org/10.1111/j.1744-313x.1991.tb00014.x
Abstract
Polymerase chain reaction (PCR) fingerprinting, a new method for the rapid matching of HLA-Dr/Dw allotypes, involves the visual comparison of polymorphic HLA-DRB gene second exon PCR products, resolved in non-denaturing polyacrylamide gels (Bidwell & Hui, 1990). We show here that the satellite DNA bands within PCR fingerprints originate by heteroduplex formation between heterologous DNAs co-amplified by a common PCR primer set. We also present two further applications of the technique which permit discrimination between unrelated HLA-DR/Dw allotypes with similar PCR fingerprints.Keywords
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