Peptides and multiple antigen peptides from Schistosoma mansoni glyceraldehyde 3‐phosphate dehydrogenase: preparation, immunogenicity and immunoprotective capacity in C57BL/6 mice

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Abstract
Four monoepitopic MAPs (MAP A, B, C and E) and one bis‐diepitopic MAP B‐E derived from the primary sequence of Schistosoma mansoni glyceraldehyde 3‐phosphate dehydrogenase, previously tested in BALB/c mice, were examined for their immunogenicity and protective capacity in C57BL/6 mice. Despite multimerization into MAPs, MAP A and MAP C were poorly immunogenic. In contrast to BALB/c mice, MAP E was non‐immunogenic in C57BL/6 mice. Peptide B in the form of MAP B or bis‐diepitopic MAP B‐E elicited immune responses in C57BL/6 mice that were associated with a significant decrease in worm burden. The MAPs were prepared by the stepwise solid‐phase peptide synthesis using Boc/Bzl chemistry, successfully purified on the RP‐HPLC column and characterized by RP‐HPLC, HPCE and MALDI‐TOF MS techniques. A general strategy for MAPs purification is discussed here and the purification of MAP B and MAP E is documented in detail. Copyright © 2004 European Peptide Society and John Wiley & Sons, Ltd.