A METHOD FOR THE QUANTITATIVE DETERMINATION OF BACTERIOPHAGE
Open Access
- 20 May 1930
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 13 (5) , 557-564
- https://doi.org/10.1085/jgp.13.5.557
Abstract
In a study of the reaction between anti-staphylococcus bacteriophage and a susceptible strain of Staph. aureus, it was found that the time required to reduce a set concentration of bacteria contained in a unit volume of bacteriophage-bacteria mixture, to a particular turbidity end-point is a function of the number of bacteriophage units present at the initiation of the process. Over a wide range, plot of time against log bacteriophage units is a straight line; this plot is used as a reference standard. The bacteriophage of an unknown is determined by finding the time required to reach the turbidity end-point and reading off the corresponding log bacteriophage units on the standard curve. Accuracy is well within [plus or minus] 5%.This publication has 0 references indexed in Scilit: