Combining Flow Injection Analysis and Immobilized Enzymes for Rapid and Accurate Determination of Serum Glucose

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Abstract
Serum samples were analysed for their glucose content using a co-immobilized glucose oxidase-peroxidase bioreactor and flow injection analysis. The enzymes were covalently coupled to a 2-fluoro-1-methylpyridinium activated support under mild and facile conditions. Optimum activity was achieved when a total of 0.72 mg protein per mL of support and in the ratio of 1.8 to 1.0 (glucose oxidase :peroxidase, in terms of their activities) was offered for coupling. The immobilized enzyme showed excellent storage stability at 4O °C retaining up to 95% of its initial activity after 60 days.