Glutamic oxaloacetic transaminase isoenzymes in bovine liver disease.

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Abstract
Serum total glutamic oxaloacetic transaminase (t-GOT), mitochondrial GOT (m-GOT)and glutamate dehydrogenase (GLDH) activities were determined in 26 calves and 86 cows as healthy controls and in 232 cases of liver disorders. In addition, the changes in GOT isoenzymes were examined in 4 calves experimentally induced liver damage. GOT isoenzymes were separated both by a simplified column chromatographic method coupled with UV assay and by an enzyme-electrophoretic method. The m-GOT activities of healthy controls by the chromatographic method were about three times higher than those by the electrophoretic method. Clinically, the electrophoretic method was proved to be more practical than the chromatographic method in bovine. The cleaf rise and fall in serum m-GOT activity indicated that the m-GOT and the m-GOT/t-GOT ratio were sensitive markers for experimentally induced hepatic lesions at acute stages in cows. In the clinical cases, m-GOT increased moderately or remarkably in hepatocellular necrosis, liver fibrosis, tumor of the liver and amyloidosis, but it showed little increase in hydropic droplet degeneration, fascioliasis and fatty change of the liver. As the m-GOT/t-GOT ratio had less effect on muscular damage in cows than the individual t-GOT and m-GOT, the ratio was thought to be a very useful diagnostic aid in clinical cases.

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