A Low‐Dose Immunization Schedule for the Production of ALG in Rabbits.

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Abstract
ALG was produced in rabbits by intracutaneous injections of small doses (106 cells) of C3H mouse thymocytes suspended in Freund's incomplete adjuvant. The immunosuppressive potency of this ‘low‐dose’ ALG evaluated by allo‐skin graft survival proved to be comparable to that of ALG produced by intravenous injection of high doses of lymphoid cells (ad modum Levey & Medawar). In addition the ‘low‐dose’ method gave rise to negligible amounts of haemagglutinins, lymphagglutinins, and lymphocytotoxic antibodies. The ‘low‐dose’ method may represent a major advantage in overcoming the limiting factor presented by scarcity of available antigen, which is regularly the case in human ALG production.