CLONED MOUSE MAST-CELLS DERIVED FROM IMMUNIZED LYMPH-NODE CELLS AND FROM FETAL LIVER-CELLS EXHIBIT CHARACTERISTICS OF BONE MARROW-DERIVED MAST-CELLS CONTAINING CHONDROITIN SULFATE-E PROTEOGLYCAN

  • 1 January 1984
    • journal article
    • research article
    • Vol. 52  (3) , 563-575
Abstract
Cloned mouse mast cells which were T cell growth-dependent were derived both from immunized lymph node and from fetal liver, and were found to be morphologically and biochemically similar to mast cells previously differentiated in vitro from mouse bone marrow (BMMC). These 2 T cell growth-dependent mouse mast cell clones were identical to the BMMC in their preferential synthesis of chondroitin sulfate E proteoglycan rather than heparin proteoglycan. The hydrodynamic size of the cell-associated proteoglycan from each of the 3 mast cell sources was 150,000-250,000 MW; and that of the covalently bound glycosaminoglycans was 13,000-25,000 MW. Chondroitinase ABC digestion of the [35S]proteoglycans from both cloned mast cells, as well as the BMMC, yielded only 2 disaccharides which comigrated on ascending TLC with .DELTA.Di-4S [2-acetamido-2-deoxy-3-O-(.beta.-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose] and .DELTA.Di-diSE [2-acetamido-2-deoxy-3-O-(.beta.-D-gluco-4-enepyranosyluronic acid)-4-6-di-O-sulfo-D-galactose] standards, respectively. Quantification of the radioactivity in the enzyme digests revealed that 1/6 - 1/2 of the resulting disaccharides were disulfated, similar to that found in BMMC containing chondroitin sulfate E. When sensitized with monoclonal IgE, washed and subsequently challenged with specific antigen, each of the 2 cloned mast cells generated > 100 ng of leukotriene C4 (LTC4)/106 cells, but only 3-12 ng leukotriene B4 (LTB4)/106 cells, characteristics also observed for the BMMC. The cloned mast cells from lymph node and liver and the bone marrow-derived mast cells belong to a distinct subclass of mast cells. These mast cells were designated E-mast cells to distinguish them from heparin-containing mast cells.