Spectrophotometric Determination of Vitamin A by Means of Chromatography.

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Abstract
After saponification and extraction, vit. A is adsorbed on Ca3(PO4)2 by means of petroleum ether. It is advanced and eluted with a petroleum ether-ether mixture (50:1). For low concns. it is necessary to use Al2O3. Spectrophotometric measurements on these eluates gives a specific detn. of vit. A in most samples. Losses of pure vit. A due to chromatography are 0.5 to 2%. Values with and without chromatographic adsorption are given for extracts from whale oils, cod liver oils, liver paste, butter, milk, vitaminized oats and fodder mixtures. The authors suggest preference of their procedure to that of using the correction of Morton and Stubbs.

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