Factor 390 chromophores: phosphodiester between AMP or GMP and methanogenic factor 420

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Abstract
Two chromophores with absorbance maxima at 390 nm (factors 390) were isolated from oxidized cells of Methanobacterium thermoautotrophicum .DELTA.H. The isolation procedure included anion-exchange chromatography of the soluble cofactor pool followed by reverse-phase chromatography. The factor 390 species are novel derivatives of methanogen coenzyme factor 420 in which the 5-deazaflavin 8-hydroxy group is in a phosphodiester linkage to AMP or GMP. The structural assignments were based, in part, on the UV-visible and 1H NMR spectra. The results from amino acid analysis, phosphate determination, 31P NMR spectroscopy and fast atom bombardment mass spectrometry were consistent with the proposed structures. Confirmation of the factor 390 structures was made following phosphodiesterase release of the nucleotide monophosphates from factor 420. The nucleotide monophosphates were identified as AMP and GMP by UV-visible spectra and based on elution position by using reverse-phase and anion-exchange high-performance liquid chromatography. The presence of AMP was further demonstrated by using AMP kinase which induced a spectral shift during conversion of the sample to IMP. In addition, the presence of GMP was established by a specific enzymatic assay.

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