Anion exchange and volume regulation during metabolic blockade of renal cortical slices.

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Abstract
The development of swelling of rat and guinea pig renal cortical slices was studied after metabolic blockade (hypoxia plus glycolytic blockade with iodo-acetic acid) and/or exposure to isotonic high K, no Na solution. Swelling was greater after exposure to oxygenated high K solution than after metabolic blockade in physiologic Krebs-Henseleit solution. Swelling was reduced after metabolic blockade in high K solution compared to incubation in oxygenated high K solution. Increasing periods of transient metabolic blockade in Krebs-Henseleit solution progressively blunted swelling when slices were subsequently incubated in oxygenated high K solution. Metabolic blockade in Krebs-Henseleit solution resulted in marked reductions in K and increases in Na. Incubation in high K solution resulted in marked increases in K and similar low levels of Na regardless of associated interventions. Metabolic blockade in both media resulted in significantly greater increases in renal cortical chloride than in monovalent cations (K plus Na). Incubation in oxygenated high K solution was associated with similar increases in renal cortical chloride and total monovalent cations. Renal cortical losses of solids and protein and increases in renal cortical inulin space were greater after metabolic blockade than after incubation under oxygenated conditions regardless of the incubation media. During metabolic blockade there is a significant replacement of larger intracellular anions by extracellular chloride. The loss of osmotically active intracellular anions limits the increase in renal cortical volume during metabolic inhibition and exposure to high K solution.