NMR Study of 100 kDa HCV IRES RNA Using Segmental Isotope Labeling
- 20 July 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 124 (32) , 9338-9339
- https://doi.org/10.1021/ja026647w
Abstract
RNA NMR is hindered by the large size of most biological RNAs. We present here a simple method for segmental isotopic labeling of an RNA fragment within the context of a larger RNA. The methodology uses transcription and ribozyme cleavage to prepare appropriate ends for RNA ligase catalyzed ligation. We demonstrate that a 64 nucleotide domain of the Hepatitis C virus internal ribosome entry site (IRES) RNA adopts an independently folded domain within the context of the intact, 100 kDa IRES.Keywords
This publication has 7 references indexed in Scilit:
- The internal ribosome entry site (IRES) of hepatitis C virus visualized by electron microscopyRNA, 2001
- Hepatitis C Virus IRES RNA-Induced Changes in the Conformation of the 40 S Ribosomal SubunitScience, 2001
- TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteinsProceedings of the National Academy of Sciences, 1998
- Stable Isotope-Edited NMR Analysis of Ascaris suum Mitochondrial tRNAMet Having a TV-Replacement LoopThe Journal of Biochemistry, 1998
- Direct Measurement of Distances and Angles in Biomolecules by NMR in a Dilute Liquid Crystalline MediumScience, 1997
- Crystallization of RNA-protein complexes I. Methods for the large-scale preparation of RNA suitable for crystallographic studiesJournal of Molecular Biology, 1995
- Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroidNucleic Acids Research, 1986