Structural Studies on the Anti-tumor Active Polysaccharides from Coriolus versicolor (Basidiomycetes). II. Structures of β-D-Glucan Moieties of Fractionated Polysaccharides

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Abstract
Structural examination was made on the glucan moiety of the 3 fractions composed of .beta.-D-glucan and 12-14% protein, among the 5 fractions of the water-soluble polysaccharide mixture obtained from the mycelium of C. versicolor. On periodate oxidation, each fraction consumed about 1.5 mol of the oxidant with formation of about 0.5 mol of formic acid. Periodate oxidation followed by reduction with potassium borohydride and subsequent hydrolysis of each fraction gave glycerol, erythritol and glucose. Complete methylation and hydrolysis afforded 2,3,4,6-tetra-, 2,3,6-tri-, 2,3-di-, 2,4-di and 2,6-di-O-methyl-D-glucoses in the proportion different for each fraction. Identification of these hydrolysis products was carried out on the crystalline basis as well as by GLC. The glucan moiety of the fraction with highest antitumor activity was composed of 1 .fwdarw. 4 linked .beta.-D-glucose residues, which were occasionally branched at 3- and 6-positions and that 2 other fractions with lower antitumor activity had a more complicated ramified glucans with increasing proportions of 1-3 and 1-6 linkages.