Destruction of Rabbit Platelets in the Allergic Response of Sensitized Leukocytes

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Abstract
The interaction of antigen with sensitized rabbit leukocytes results in histamine release and is cytotoxic for platelets in the reaction mixtures. The destruction of the platelets requires no exogenous serum factors and is proportional to the number of sensitized leukocytes in the reaction mixtures. Destruction of the platelets follows the release of one or more soluble intermediate(s) when the sensitized leukocytes react with antigen. The release of this factor(s) from leukocytes requires Ca2+ (optimal at 3 × 10-4 M) and is inhibited by Mg2+. The action of this factor on platelets is temperature sensitive and is essentially complete in 5 min. The activity required for platelet destruction is also mediated by Ca2+ and is not inhibited by Mg2+. These observations suggest that the histamine release from sensitized rabbit leukocytes is associated with extracellular appearance of other, as yet unidentified substances whose activity is both temperature and calcium dependent.

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