Comparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis in separating Escherichia coli uidA amplicons differing in single base substitutions
Open Access
- 1 June 2000
- journal article
- research article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 30 (6) , 427-431
- https://doi.org/10.1046/j.1472-765x.2000.00742.x
Abstract
A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for...Keywords
This publication has 14 references indexed in Scilit:
- Simultaneous Detection and Differentiation of Escherichia coli Populations from Environmental Freshwaters by Means of Sequence Variations in a Fragment of the β- d -Glucuronidase GeneApplied and Environmental Microbiology, 2000
- Germline PTEN mutations in Cowden syndrome-like families.Journal of Medical Genetics, 1998
- Mutation analyses of KRAS exon 1 comparing three different techniques: temporal temperature gradient electrophoresis, constant denaturant capillaryelectrophoresis and allele specific polymerase chain reactionMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1998
- Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecologyAntonie van Leeuwenhoek, 1998
- Evaluation of denaturing gradient gel electrophoresis in the detection of 16S rDNA sequence variation in rhizobia and methanotrophsFEMS Microbiology Ecology, 1997
- Genetic testing: The problems and the promiseNature Biotechnology, 1997
- [4] Use of denaturing gradient gel electrophoresis to study conformational transitions in nucleic acidsPublished by Elsevier ,1992
- Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.Proceedings of the National Academy of Sciences, 1989
- [30] Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresisPublished by Elsevier ,1987
- Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresisNucleic Acids Research, 1985