myo‐Inositol 1,4,5‐trisphosphorothioate is a potent competitive inhibitor of human erythrocyte 5‐phosphatase
- 2 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 242 (2) , 373-377
- https://doi.org/10.1016/0014-5793(89)80504-6
Abstract
The effect of the myo-inositol 1,4,5-trisphosphate (IP3) analogue, myo-inositol 1,4,5-trisphosphorothioate (IPS3) on the dephosphorylation of D-5-[32P]IP3 by the 5-phosphatase from human erythrocyte membranes has been investigated. DL-IPS3 was found to act as a competitive inhibitor with a Ki of 6 μM, making it the most potent inhibitor currently available for this enzyme. L-IP3 inhibited the enzyme with a Ki of 124 μM and was more potent than D-2,3-diphosphoglycerate (Ki 978 μM)Keywords
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