The regulatory region of phage fr replicase cistron. III. Initiation activity of specific fr RNA fragments
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (23) , 7763-7775
- https://doi.org/10.1093/nar/10.23.7763
Abstract
RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related RNA from phage MS2 have allowed us to pinpoint the boundaries of the replicase cistron initiation region on phage RNA. A structural model of the above initiation region has been provided which shows that besides the minimal initiation region (comprises the Shine-Dalgarno sequence and initiator AUG), the flanking regions are also involved and are responsible for additional interactions with the ribo-some. The flanking regions possibly contribute to the stability of specific contact between the ribosome and template realized by the minimal initiation region.Keywords
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