Quantitation of Chill-Induced Release of a Tubulin-Like Factor and Its Prevention by Abscisic Acid in Gossypium hirsutum L.

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Abstract
The degree of tubulin polymerization in cotton (Gossypium hirsutum L. cv Acala) cotyledonary tissue was estimated by radioimmunoassay which measured the amount of a tubulin-like factor. It was assumed that the release of this tubulin-like factor indicated depolymerization of microtubules. Exposure to chilling resulted in complete release of the tubulin-like factor. Pretreatment with abscisic acid in the light almost completely prevented the chill-induced release of the tubulin-like factor. Addition of colchicine during the chilling period accelerated the release of the tubulin-like factor. Pretreatment with abscisic acid greatly reduced this effect of colchicine. It is concluded that the destruction of the microtubular network is involved in the development of chilling injury in cotton. Abscisic acid apparently decreased chilling injury by stabilization of the microtubular network.