2 MONOCLONAL-ANTIBODIES (DLY-1 AND DLY-6) DIRECTED AGAINST CANINE LYMPHOCYTES

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 10  (7) , 609-619
Abstract
Two murine monoclonal antibodies directed against canine lymphocytes are described. DLy-1, raised against puppy thymocytes, and DLy-6, raised against bronchoalveolar lymphocytes, both reacted with most lymphocytes in peripheral blood, thoracic duct lymph and bronchoalveolar lavage fluids. DLy-1 also recognized monocytes and granulocytes. It was not reactive with erythrocytes, megakaryocytes or platelets. Expression of DLy-1 antigen on thymocytes ranged from 5-30%. The distribution of DLy-6 antigens seems to be confined to lymphoid cells. Ten to 60% puppy thymocytes were positive. Lymphoblasts formed in response to stimulation with mitogens or alloantigens lacked DLy-6 in contrast to DLy-1 cell surface antigen expression.