DIMENSIONAL, OSMOTIC, AND CHEMICAL CHANGES OF ERYTHROCYTES IN STORED BLOOD. IV. CELLS SEPARATED FROM PLASMA 1

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Abstract
Even under the best conditions, the preservation of separated red cells is inferior and much more variable than that of the cells of corresponding whole blood. Cells derived from bloods collected in ACD soln. are better preserved than those derived from bloods collected in citrate soln. The preservation of cells from citrate blood may be improved by resuspension in acidified solns., but even then they are not so well preserved as ACD cells. The optimal acidity for the preservation of citrated cells may be defined within the limits of 0.14-0.35 mM. of citric acid/100 ml. of cells. ACD cells may be conveniently and advantageously stored in packed form. The cause of the inferior preservation of separated red cells is unknown at present. Addition of globulin or gelatin products does not appear to improve their preservation.