Construction of a Hybrid Plasmid Molecule Containing the Total Coding Region of Semliki Forest Virus 26S mRNA
- 1 February 1982
- journal article
- letter
- Published by Microbiology Society in Journal of General Virology
- Vol. 58 (2) , 443-448
- https://doi.org/10.1099/0022-1317-58-2-443
Abstract
A hybrid plasmid molecule was constructed which contains the complete coding sequences from the 26S mRNA of Semliki Forest virus. Five fragments which together covered the mRNA sequence were isolated from 3 original hybrid plasmids and joined together at 5 different restriction endonuclease cleavage sites using T4 DNA ligase.This publication has 5 references indexed in Scilit:
- The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region.Proceedings of the National Academy of Sciences, 1980
- Nucleotide sequence of cDNA coding for Semliki Forest virus membrane glycoproteinsNature, 1980
- Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globinCell, 1980
- A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gelsAnalytical Biochemistry, 1979
- A bacterial clone synthesizing proinsulin.Proceedings of the National Academy of Sciences, 1978