Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography
- 1 July 1981
- Vol. 16 (7) , 541-545
- https://doi.org/10.1007/bf02535053
Abstract
A method is described for the separation and analysis of phospholipids from rat-liver nuclear envelope and endoplasmic reticulum. The procedure employs a liquid environment, to which antioxidants can be added, and results in separation of neutral lipids (NL), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), and phosphatidylcholine (PC) in 99% purity in 12 min; analytical columns and a radial compression system may be employed. The procedure results in phospholipids with a large proportion of highly unsaturated fatty acids; some differences in fatty acid distributions were found when nuclear envelope phospholipid fractions were compared with the corresponding fractions from endoplasmic reticulum.This publication has 13 references indexed in Scilit:
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