CYTOFLUOROMETRIC METHOD OF COUNTING TRYPANOSOMES

  • 1 January 1978
    • journal article
    • research article
    • Vol. 29  (1) , 95-100
Abstract
A rapid automated method of counting trypanosomes was developed using cytofluorometric methods. Organisms were vitally stained with acridine orange, and DEAE cellulose separated Trypanosoma congolense were accurately counted at all concentrations. T. congolense in heparinized whole bovine blood were also counted accurately at levels greater than 1000/.mu.l. At lower levels, small fluorescent particles in whole blood interfered with the counts.

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