Antioxidant and Lipophilic Constituents ofTinospora crispa

Abstract
TLC autographic assays revealed in the CH2Cl2 extract of Tinospora crispa Miers (Menispermaceae) the presence of three compounds exhibiting antioxidant and radical scavenging properties towards β-carotene and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. They were isolated and identified as N-cis-feruloyltyramine (4), (N-trans-feruloyltyramine (6) and secoisolariciresinol (5). When tested in dilution assays on the reduction of the DPPH radical, these 3 compounds proved to be more active than the synthetic antioxidant butylhydroxytoluene (BHT). Further investigation of the CH2Cl2 extract led to the isolation of vanillin, syringin, the alkaloid N-formylnornuciferin and the diterpene derivatives borapetosides B and C. In addition, a LC/UV/MS analysis enabled the on-line identification of borapetoside F and N-formylannonain.

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