Utility of Ethidium Bromide in the Extraction from Whole Plants of High Molecular Weight Maize DNA
Open Access
- 1 December 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 66 (6) , 1140-1143
- https://doi.org/10.1104/pp.66.6.1140
Abstract
A procedure is reported for the isolation of high molecular weight maize DNA from whole plant tissue. Nuclei are isolated in the presence of ethidium bromide from leaf, node, and tassel or endosperm tissues and the DNA is extracted and purified. The resulting DNA has a double-strand molecular weight of about 125 kilobase pairs and a single-strand molecular weight of about 125 kilobases. The DNA is cleavable by a number of common restriction endonucleases.This publication has 29 references indexed in Scilit:
- Nucleotide Sequence Specificity of Restriction EndonucleasesScience, 1979
- Dominant lethality in frog embryos after paternal treatment with triethylenemelamine: Cytogenetics, morphology, and swimming capabilityEnvironmental Mutagenesis, 1979
- Cloning of the yeast ribosomal DNA repeat unit in SstI and HindIII lambda vectors using genetic and physical size selectionsJournal of Molecular Biology, 1978
- The isolation of high molecular weight DNA from whole organisms or large tissue massesAnalytical Biochemistry, 1978
- Separation of very large DNA molecules by gel electrophoresisNucleic Acids Research, 1978
- Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA CloningScience, 1977
- Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.Proceedings of the National Academy of Sciences, 1977
- Isolation and Properties of Deoxyribonucleic Acid from Protoplasts of Cell Suspension Cultures of Ammi visnaga and Carrot (Daucus carota)Plant Physiology, 1972
- Mass Isolation of Pea NucleiPlant Physiology, 1971
- MOLECULAR SIZE AND CIRCULARITY OF DNA IN CELLS OF MAMMALS AND HIGHER PLANTSProceedings of the National Academy of Sciences, 1965