Effect of Osmolality on Aldosterone Secretion*

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Abstract
Whether the powerful effect of NaCl concentration on angiotensin II- and K-stimulated aldosterone secretion by isolated perfused adrenal glands is mediated by the Na+ or Cl- ion or by the obligatory change in osmolality was investiated. Isolated Ringer''s bicarbonate perfused canine adrenal gland preparations were to determine the effects of a variety of isosmotic, hyperosmotic, and hyposmotic solutions on angiotensin II and K-stimulated aldosterone secretion. When the osmolality of the perfusion medium (8-10 mosmol) was increased by the addition of NaCl, sucrose, mannitol, or glucose, angiotensin II-stimulated aldosterone secretion was inhibited to a similar extent, whereas urea addition had no effect. Similarly, when increased in the osmolality of the perfusion medium (8-10 mosm) was by the addition of NaCl, sucrose, or mannitol, K-stimulated aldosterone secretion was also inhibited to a similar extent. In contrast to the increase in angiotensin II- and K-stimulated aldosterone secretion observed during hyposmotic reductions in NaCl concentration, (addition of sucrose) did not increase angiotensin II- or K-stimulated aldosterone secretion. Even the marked increase in aldosterone secretion caused by large hyposmotic reduction in NaCl concentration did not occur with an equivalent isosmotic reduction in NaCl concentration. These results clearly demonstrate that changes in NaCl concentration affect aldosterone secretion by a mechanism sensitive to the osmolality. Moreover, since hyperosmolality caused by urea addition had no effect on angiotensin II-stimulated aldosterone secretion, changes in intracellular volume or composition appear to be an important modulator of aldosterone secretion.